Journal: Scientific Reports

Article Title: SETD4-expressing cells contribute to pancreatic development and response to cerulein induced pancreatitis injury

doi: 10.1038/s41598-021-92075-5

Figure Lengend Snippet: Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + ) pancreatic cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional Matrigel. Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.

Article Snippet: After gelation of the Matrigel, pancreatic organoid growth medium (Stem Cell Technologies #06040) was added in each well and replenished every 3 days.

Techniques: Immunofluorescence, Clone Assay, Recombinant, Isolation, Fluorescence, FACS, Cell Culture, Staining

Journal: Frontiers in Microbiology

Article Title: Disclosing the benefits of multi-strain compounds and their health impact mechanisms utilizing intestinal biomimetic technology

doi: 10.3389/fmicb.2025.1550913

Figure Lengend Snippet: Construction of the human colonic organoid culture model. (a) Human colonic organoid culture model; (b) Observation of organoid growth from Day 1 to Day 7 under an optical microscope. Scale bar, 50 μm.

Article Snippet: Subsequently, 750 μL of colonic organoid growth medium (06010, STEMCELL, Shanghai, China) was added to each well.

Techniques: Microscopy